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Nitropeptide Profiling and Identification Illustrated by Angiotensin II
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June 16, 2019
DOI:
1Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 2Department of Biological Sciences,University of Notre Dame, 3Department of Urology, Shanghai East Hospital,Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases,University of Notre Dame, 5Harper Cancer Research Institute,University of Notre Dame, 6Tumor Microenvironment and Metastasis Program,Indiana University Melvin and Bren Simon Cancer Center
Summary
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Proteomic profiling of tyrosine-nitrated proteins has been a challenging technique due to the low abundance of the 3-nitrotyrosine modification. Here we describe a novel approach for nitropeptide enrichment and profiling by using Angiotensin II as the model. This method can be extended for other in vitro or in vivo systems.
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Cite this Article
Feng, S., Wen, X., Lu, X. Nitropeptide Profiling and Identification Illustrated by Angiotensin II. J. Vis. Exp. (148), e59391, doi:10.3791/59391 (2019).
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