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A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
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A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

20,482 Views

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04:19 min

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April 19, 2019

DOI:

10.3791/59295-v

DOI:
10.3791/59295-v

•

04:19 min
April 19, 2019

•

12 Views
, , , , ,
1Bayer School of Natural and Environmental Sciences,Duquesne University, 2Department of Biological Sciences,Duquesne University, 3Chronic Pain Research Consortium,Duquesne University, 4Farmasötik Bilimler Enstitüsü,Duquesne University

Özet

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Here we present a method to validate tail vein injections in rats by utilizing near-infrared fluorescence imaging data from dyes incorporated into agents or biological probes. The tail is imaged before and after the injection, the fluorescent signal is quantified, and an assessment of the injection quality is made.

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Cite this Article

Saleem, M., Stevens, A. M., Deal, B., Liu, L., Janjic, J., Pollock, J. A. A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents. J. Vis. Exp. (146), e59295, doi:10.3791/59295 (2019).

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