ジャーナル
/
医学
/
A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents
JoVE Journal
医学
This content is Free Access.
JoVE Journal 医学
A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

DOI:
10.3791/59295-v

•

04:19 min

•

April 19, 2019

•

, , , , ,
1Bayer School of Natural and Environmental Sciences,Duquesne University, 2Department of Biological Sciences,Duquesne University, 3Chronic Pain Research Consortium,Duquesne University, 4大学院製薬科学研究科,Duquesne University

章

  • 00:04標題
  • 00:47Near-infrared (NIRF)-containing Agent Tail Vein Injection
  • 03:02Results: Representative NIRF-based Nanoemulsion Tail Vein Imaging
  • 03:40Conclusion

概要

自動翻訳

自動翻訳

Here we present a method to validate tail vein injections in rats by utilizing near-infrared fluorescence imaging data from dyes incorporated into agents or biological probes. The tail is imaged before and after the injection, the fluorescent signal is quantified, and an assessment of the injection quality is made.

タグ

Tail Vein InjectionNear-infrared-labeled AgentsFluorescence ImagingAnesthetized AnimalTail VasculatureLateral Tail Vein25-27 Gauge NeedleNeedle PlacementSyringe InjectionCatheterBlood FlashbackQuality Control動物実験Reproducibility

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Screening for Melanoma Modifiers using a Zebrafish Autochthonous Tumor Model

Heterotopic Auxiliary Rat Liver Transplantation With Flow-regulated Portal Vein Arterialization in Acute Hepatic Failure

Vascular Balloon Injury and Intraluminal Administration in Rat Carotid Artery

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System

Synergizing Antegrade Endoscopic with Bridging Vein Harvesting for Improvement of Great Saphenous Vein Graft Quality from the Lower Leg

A Rat Carotid Artery Pressure-Controlled Segmental Balloon Injury with Periadventitial Therapeutic Application

Setup and Execution of the Rapid Cycle Deliberate Practice Death Notification Curriculum

A Murine Tail Lymphedema Model

Read Article