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Met behulp van Nanoplasmon-Enhanced verstrooiing en low-vergroting Microscoop Imaging te kwantificeren tumor-afgeleide Exosomes
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Met behulp van Nanoplasmon-Enhanced verstrooiing en low-vergroting Microscoop Imaging te kwantificeren tumor-afgeleide Exosomes
1Department of Integrated Traditional Chinese and Western Medicine,West China Hospital of Sichuan University, 2Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute,Arizona State University, 3School of Biological and Health Systems Engineering,Arizona State University, 4Elektrik ve Bilgisayar Mühendisliği Bölümü,North Dakota State University
Bölümler
- 00:04Başlık
- 01:01Preparation of Nanoparticle Probes (AuNR-IgG)
- 02:17Preparation of Extracellular Vesicle Capture Slides and Exosome Capture
- 05:17Low-Magnification Dark-Field Microscope (LMDFM) Image Acquisition and Analysis
- 07:48Results: Exosome Quantification by Analysis of LMDFM Images
- 08:25Conclusion
De klinische vertaling van exosoom biomarkers voor zieke en kwaadaardige cellen wordt belemmerd door het ontbreken van snelle en accurate kwantificatie methoden. Dit rapport beschrijft het gebruik van low-vergroting Dark-Field Microscoop beelden te kwantificeren specifieke exosoom subtypen in kleine volume serum of plasma monsters.
