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Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
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May 24, 2019
DOI:
1Department of Integrated Traditional Chinese and Western Medicine,West China Hospital of Sichuan University, 2Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute,Arizona State University, 3School of Biological and Health Systems Engineering,Arizona State University, 4Department of Electrical and Computer Engineering,North Dakota State University
Summary
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Clinical translation of exosome-derived biomarkers for diseased and malignant cells is hindered by the lack of rapid and accurate quantification methods. This report describes the use of low-magnification dark-field microscope images to quantify specific exosome subtypes in small volume serum or plasma samples.
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Wan, M., Amrollahi, P., Sun, D., Lyon, C., Hu, T. Y. Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes. J. Vis. Exp. (147), e59177, doi:10.3791/59177 (2019).
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