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Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

8,195 Views

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10:49 min

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January 28, 2019

DOI:

10.3791/58821-v

DOI:
10.3791/58821-v

•

10:49 min
January 28, 2019

•

14 Views
,
1Department of Civil and Environmental Engineering,Tohoku University, 2Department of Frontier Science for Advanced Environment, Graduate School of Environmental Studies,Tohoku University

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Here we present two protocols, one for measuring the specific growth rate and the other for the cell-binding ability of rotavirus using the plaque assay and RT-qPCR. These protocols are available for confirming the differences in phenotypes between rotavirus strains.

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Cite this Article

Kadoya, S., Sano, D. Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus. J. Vis. Exp. (143), e58821, doi:10.3791/58821 (2019).

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