Journal
/
Imunologia e Infecção
/
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
JoVE Journal
Imunologia e Infecção
This content is Free Access.
JoVE Journal Imunologia e Infecção
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus

8,117 Views

•

10:49 min

•

January 28, 2019

DOI:

10.3791/58821-v

DOI:
10.3791/58821-v

•

10:49 min
January 28, 2019

•

14 Views
,
1Department of Civil and Environmental Engineering,Tohoku University, 2Department of Frontier Science for Advanced Environment, Graduate School of Environmental Studies,Tohoku University

Summary

Automatically generated

Here we present two protocols, one for measuring the specific growth rate and the other for the cell-binding ability of rotavirus using the plaque assay and RT-qPCR. These protocols are available for confirming the differences in phenotypes between rotavirus strains.

Explore More Videos

RotavirusPlaque AssayCell-binding AssayMA104 Cell LineCell CultureVirus TiterDisinfectant-resistant StrainsPhenotypic ChangeVirus PropagationVirus ActivationMultiplicity Of Infection (MOI)

Read Article

Cite this Article

Kadoya, S., Sano, D. Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus. J. Vis. Exp. (143), e58821, doi:10.3791/58821 (2019).

Download .ris file

Copy

Share Video

.

Copy