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Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
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JoVE Journal İmmünoloji ve Enfeksiyon
Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

DOI:
10.3791/58664-v

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07:07 min

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January 07, 2019

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,
1Pulmonary, Immunology and Physiology Laboratory, Department of Pediatrics,Pennsylvania State University College of Medicine, 2Biyokimya ve Moleküler Biyoloji Anabilim Dalı,Pennsylvania State University College of Medicine

Bölümler

  • 00:04Başlık
  • 00:31Estrous Cycle Stage Assessment, Ozone Exposure, and Lung Collection
  • 02:11RNA Preparation
  • 03:43microRNA (miRNA) Profiling
  • 04:40Results: Representative Functional microRNA Analyses
  • 06:26Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here we describe a method to assess lung expression of miRNAs that are predicted to regulate inflammatory genes using mice exposed to ozone or filtered air at different stages of the estrous cycle.

Etiketler

MicroRNALungEstrous CycleOzoneMouseInflammationVaginal FluidRNA ExtractionReal-time PCR

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