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Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
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JoVE Journal 免疫学と感染
Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

DOI:
10.3791/58664-v

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07:07 min

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January 07, 2019

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1Pulmonary, Immunology and Physiology Laboratory, Department of Pediatrics,Pennsylvania State University College of Medicine, 2生化学・分子生物学科,Pennsylvania State University College of Medicine

章

  • 00:04標題
  • 00:31Estrous Cycle Stage Assessment, Ozone Exposure, and Lung Collection
  • 02:11RNA Preparation
  • 03:43microRNA (miRNA) Profiling
  • 04:40Results: Representative Functional microRNA Analyses
  • 06:26Conclusion

概要

自動翻訳

自動翻訳

Here we describe a method to assess lung expression of miRNAs that are predicted to regulate inflammatory genes using mice exposed to ozone or filtered air at different stages of the estrous cycle.

タグ

MicroRNALungEstrous CycleOzoneMouseInflammationVaginal FluidRNA ExtractionReal-time PCR

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