Dergi
/
Nörobilim
/
监测小鼠睡眠/觉醒状态期间神经回路的光遗传学操作
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Optogenetic Manipulation of Neural Circuits During Monitoring Sleep/wakefulness States in Mice
Please note that all translations are automatically generated. Click here for the English version.

监测小鼠睡眠/觉醒状态期间神经回路的光遗传学操作

DOI:
10.3791/58613-v

•

08:58 min

•

June 19, 2019

•

, ,
1Department of Molecular Neuroscience and Integrative Physiology, Faculty of Medicine,Kanazawa University, 2International Institute for Integrative Sleep Medicine (WPI-IIIS),University of Tsukuba, 3Faculty of Medicine,University of Tsukuba

Bölümler

  • 00:04Başlık
  • 00:38Animal Preparation
  • 01:33Virus Injection
  • 02:47Electroencephalogram and Electromyogram (EEG/EMG) Electrode and Optical Fiber Implantation
  • 05:20EEG/EMG Monitoring
  • 07:19Results: Representative Effect of GABABNST Stimulation on State Transition in NREM and REM Sleep
  • 08:09Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

在这里,我们描述了在监测小鼠睡眠/觉醒状态期间对特定类型神经元的光遗传学操作方法,并举例说,我们最近对纹状体末期骨细胞核的床核进行了实验。

Etiketler

Optogenetic ManipulationNeural CircuitsSleep/wakefulnessMiceNeuro CircuitSleep Wakefulness RegulationSurface EEGAnesthetized MouseStereotactic ApparatusAdeno-associated Virus VectorMicroinjectionBed Nucleus Of The Stria Terminalis (BNST)Electroencephalogram (EEG)Electromyogram (EMG)

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

灭绝培训在彭兰霞窗口无法还原的恐惧

同时脑电图，实时测量的啮齿动物的大脑皮质中的神经元活动的乳酸浓度与Optogenetic的操作

多单位记录方法来描述蝗虫的神经活动（<em> Schistocerca美洲</em>）嗅觉电路

在体内双光子成像的经验依赖的分子在大脑皮质神经元的变化

光遗传学的扰动激光照射半完好的神经活动<em>果蝇</em>幼虫运动

在脑片恐惧电路体外光遗传学解剖

Optogenetic 在行为小鼠海马θ振荡中的作用

酒精中毒在认知控制过程中对前小管神经同步的破坏

小鼠神经干细胞和脑中5-羟基甲基细胞氨酸的检测

测量幼儿期午睡期间睡眠依赖记忆巩固背后的神经机制

Read Article