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小角 x 線散乱による溶液中の高分子の構造研究

JoVE Journal
Biyokimya

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JoVE Journal Biyokimya

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

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小角 x 線散乱による溶液中の高分子の構造研究

12,152 Views

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07:19 min

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November 05, 2018

DOI:

10.3791/58538-v

DOI:

10.3791/58538-v

•

07:19 min

November 05, 2018

•

7 Views

Tyler Mrozowich¹, Steffane McLennan², Michael Overduin^2,4, Trushar R. Patel^1,3,4

¹Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry,University of Lethbridge, ²Department of Biochemistry,Alberta Üniversitesi, ³Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine,University of Calgary, ⁴DiscoveryLab, Faculty of Medicine & Dentistry,Alberta Üniversitesi

Özet

ここでは、小角 x 線散乱 (SAXS) を高分子の構造を表す低解像度の封筒に情報を取得する利用できる方法を提案します。X 線結晶構造解析や核磁気共鳴などの高分解能構造技術と組み合わせて使用すると、小角はマルチドメイン蛋白質および高分子複合体の溶液に詳細な洞察力を提供できます。

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