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Biochemistry

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JoVE Journal Biochemistry
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
 

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering

Article DOI: 10.3791/58538-v • 07:19 min • November 5th, 2018
November 5th, 2018 •
1, 2, 2,4, 1,3,4
1Alberta RNA Research and Training Institute, Department of Chemistry and Biochemistry, University of Lethbridge, 2Department of Biochemistry, University of Alberta, 3Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary, 4DiscoveryLab, Faculty of Medicine & Dentistry, University of Alberta

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Here, we present how Small Angle X-Ray Scattering (SAXS) can be utilized to obtain information on low-resolution envelopes representing the macromolecular structures. When used in conjunction with high-resolution structural techniques such as X-Ray Crystallography and Nuclear Magnetic Resonance, SAXS can provide detailed insights into multidomain proteins and macromolecular complexes in-solution.

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Structural Studies Macromolecules Solution Small Angle X-Ray Scattering Biochemistry Size Shape Conformational Changes Biomolecules Complexes Physiologically Relevant Buffer Conditions Complimentary Technique Biophysical Methods Structural Biology Methods Structure-based Therapeutics Nanoparticals Drug Delivery Vesicles Raw Scattering Data Low Resolution Structures Data Analysis Software Packages SCATTER BioXTAS RAW ATSAS Suite
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