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口服抗生素治疗小鼠肠道微生物群的定量聚合酶链反应分析
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
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口服抗生素治疗小鼠肠道微生物群的定量聚合酶链反应分析

12,380 Views

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08:33 min

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November 17, 2018

DOI:

10.3791/58481-v

DOI:
10.3791/58481-v

•

08:33 min
November 17, 2018

•

14 Views
, ,
1The Peter Gorer Department of Immunobiology,King’s College London, 2The Francis Crick Institute

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Automatically generated

在这里, 我们提供了详细的方案, 口服抗生素给小鼠, 收集粪便样本, dna 提取和定量的粪便细菌的 qpcr。

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Jimeno, R., Brailey, P. M., Barral, P. Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment. J. Vis. Exp. (141), e58481, doi:10.3791/58481 (2018).

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