Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Rebeca Jimeno; Phillip M. Brailey; Patricia Barral

doi:10.3791/58481

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免疫学と感染

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Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

JoVE Journal
免疫学と感染

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JoVE Journal 免疫学と感染

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

12,414 Views

•

08:33 min

•

November 17, 2018

DOI:

10.3791/58481-v

DOI:

10.3791/58481-v

•

08:33 min

November 17, 2018

•

14 Views

Rebeca Jimeno^1,2, Phillip M. Brailey^1,2, Patricia Barral^1,2

¹The Peter Gorer Department of Immunobiology,King’s College London, ²The Francis Crick Institute

概要

Here we provide detailed protocols for the oral administration of antibiotics to mice, collection of fecal samples, DNA extraction and quantification of fecal bacteria by qPCR.

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Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

概要

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