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Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
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Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

50,286 Views

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10:50 min

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November 02, 2018

DOI:

10.3791/58421-v

DOI:
10.3791/58421-v

•

10:50 min
November 02, 2018

•

16 Views
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1UNC Neuroscience Center, 2UNC/NC State Joint Department of Biomedical Engineering,UNC, 3Xona Microfluidics, LLC

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This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.

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Nagendran, T., Poole, V., Harris, J., Taylor, A. M. Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons. J. Vis. Exp. (141), e58421, doi:10.3791/58421 (2018).

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