This content is Free Access.
Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons
章
- 00:04標題
- 01:27Preparation and Coating of the Multicompartment Chips
- 03:57Seeding Neurons into the Multicompartment Chips
- 05:03Fluidic Isolation of the Axonal Compartment within the Chip
- 05:49Performing Axotomy Within the Chip
- 06:36Fluorescence Immunostaining within the Chip
- 09:03Results: Neuronal Growth Comparison within Multicompartment PDMS Devices and Plastic Chips
- 10:21Conclusion
This protocol describes the use of plastic chips to culture and compartmentalize primary murine neurons. These chips are preassembled, user-friendly, and compatible with high-resolution, live, and fluorescence imaging. This protocol describes how to plate rat hippocampal neurons within these chips and perform fluidic isolation, axotomy and immunostaining.
