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Visualiser les Drosophila jambe axones des motoneurones à travers la cuticule de l’adulte
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JoVE Journal Gelişim Biyolojisi
Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle
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Visualiser les Drosophila jambe axones des motoneurones à travers la cuticule de l’adulte

9,060 Views

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08:33 min

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October 30, 2018

DOI:

10.3791/58365-v

DOI:
10.3791/58365-v

•

08:33 min
October 30, 2018

•

8 Views
, , , ,
1Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, 2Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute,Columbia University, 3Neuroscience Program,NYU School of Medicine

Özet

Automatically generated

Nous décrivons ici un protocole afin de visualiser le ciblage axonale avec une protéine fluorescente dans jambes adultes de la drosophile par fixation, montage, imagerie et imageries après étapes.

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DrosophilaLegMotor NeuronAxonsAdult CuticleFluorescent ProteinsAxon Visualization3D ReconstructionCarbon Dioxide AnesthesiaEthanolNon-ionic DetergentParaformaldehydeGlycerolMounting MediumMicroscope SlideCoverslip488 Nm Laser

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Cite this Article

Guan, W., Venkatasubramanian, L., Baek, M., Mann, R. S., Enriquez, J. Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle. J. Vis. Exp. (140), e58365, doi:10.3791/58365 (2018).

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