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Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle
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発生生物学
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JoVE Journal 発生生物学
Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

9,060 Views

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08:33 min

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October 30, 2018

DOI:

10.3791/58365-v

DOI:
10.3791/58365-v

•

08:33 min
October 30, 2018

•

8 Views
, , , ,
1Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, 2Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute,Columbia University, 3Neuroscience Program,NYU School of Medicine

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Here we describe a protocol to visualize the axonal targeting with a florescent protein in adult legs of Drosophila by fixation, mounting, imaging, and post-imaging steps.

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DrosophilaLegMotor NeuronAxonsAdult CuticleFluorescent ProteinsAxon Visualization3D ReconstructionCarbon Dioxide AnesthesiaEthanolNon-ionic DetergentParaformaldehydeGlycerolMounting MediumMicroscope SlideCoverslip488 Nm Laser

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Guan, W., Venkatasubramanian, L., Baek, M., Mann, R. S., Enriquez, J. Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle. J. Vis. Exp. (140), e58365, doi:10.3791/58365 (2018).

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