Journal
/
Biología del desarrollo
/
Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

9,060 Views

•

08:33 min

•

October 30, 2018

DOI:

10.3791/58365-v

DOI:
10.3791/58365-v

•

08:33 min
October 30, 2018

•

8 Views
, , , ,
1Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, 2Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute,Columbia University, 3Neuroscience Program,NYU School of Medicine

Summary

Automatically generated

Here we describe a protocol to visualize the axonal targeting with a florescent protein in adult legs of Drosophila by fixation, mounting, imaging, and post-imaging steps.

Explore More Videos

DrosophilaLegMotor NeuronAxonsAdult CuticleFluorescent ProteinsAxon Visualization3D ReconstructionCarbon Dioxide AnesthesiaEthanolNon-ionic DetergentParaformaldehydeGlycerolMounting MediumMicroscope SlideCoverslip488 Nm Laser

Read Article

Cite this Article

Guan, W., Venkatasubramanian, L., Baek, M., Mann, R. S., Enriquez, J. Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle. J. Vis. Exp. (140), e58365, doi:10.3791/58365 (2018).

Download .ris file

Copy

Share Video

.

Copy