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Detection and Isolation of Apoptotic Bodies to High Purity
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Detection and Isolation of Apoptotic Bodies to High Purity

Detection and Isolation of Apoptotic Bodies to High Purity

DOI:
10.3791/58317-v

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12:17 min

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August 12, 2018

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, ,
1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science,La Trobe University

Bölümler

  • 00:00Başlık
  • 00:53Induction of Apoptosis
  • 02:34Apoptotic Body Isolation using FACS
  • 04:22ApoBD Isolation via Differential Centrifugation
  • 06:02Flow Cytometry Gating Strategy
  • 10:56Representative Result: Validation of Apoptosis
  • 11:16Representative Result: Comparison of ApoBD-Enriched Sample Purity
  • 11:47Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

A workflow using flow cytometry or differential centrifugation is developed to detect, quantify and isolate apoptotic bodies from an apoptotic sample to high purity.

Etiketler

Apoptotic BodiesExtracellular VesiclesHematosisCell ClearanceIntracellular CommunicationApoptosis InductionUV IrradiationApoptotic MorphologiesApoptotic BlebbingFACSAnnexin VTOPO-3Cell StrainerCell LinesTHB1 Monocytes

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