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Detection and Isolation of Apoptotic Bodies to High Purity
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Bioquímica
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JoVE Revista Bioquímica
Detection and Isolation of Apoptotic Bodies to High Purity

Detection and Isolation of Apoptotic Bodies to High Purity

DOI:
10.3791/58317-v

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12:17 min

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August 12, 2018

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, ,
1Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science,La Trobe University

Capítulos

  • 00:00Título
  • 00:53Induction of Apoptosis
  • 02:34Apoptotic Body Isolation using FACS
  • 04:22ApoBD Isolation via Differential Centrifugation
  • 06:02Flow Cytometry Gating Strategy
  • 10:56Representative Result: Validation of Apoptosis
  • 11:16Representative Result: Comparison of ApoBD-Enriched Sample Purity
  • 11:47Conclusion

Summary

Traducción Automática

Traducción Automática

A workflow using flow cytometry or differential centrifugation is developed to detect, quantify and isolate apoptotic bodies from an apoptotic sample to high purity.

Tags

Apoptotic BodiesExtracellular VesiclesHematosisCell ClearanceIntracellular CommunicationApoptosis InductionUV IrradiationApoptotic MorphologiesApoptotic BlebbingFACSAnnexin VTOPO-3Cell StrainerCell LinesTHB1 Monocytes

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