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分離と全体ブタ膵臓の Decellularization
JoVE Journal
Biyomühendislik
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JoVE Journal Biyomühendislik
Isolation and Decellularization of a Whole Porcine Pancreas
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分離と全体ブタ膵臓の Decellularization

9,104 Views

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09:08 min

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October 10, 2018

DOI:

10.3791/58302-v

DOI:
10.3791/58302-v

•

09:08 min
October 10, 2018

•

16 Views
, , ,
1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy,University of Gothenburg, 2The Transplant Institute,Sahlgrenska University Hospital

Özet

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全体膵臓の組織工学は、外分泌と内分泌機能のための挑戦です。そのままブタ膵臓と界面活性剤 Triton X-100 の灌流成功 decellularization のプロセス、デオキシ コール酸ナトリウム、デオキシリボヌクレアーゼの解剖のための手法を示す.

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Keyword Extraction:Porcine PancreasDecellularizationDissectionBlood VesselsPeristaltic PumpOrgan ChamberDetergent ContainerDetergent Collection ContainerHeparinizedFemale PigMidline IncisionSplenic LobeDuodenal LobeConnection LobeMajor Duodenal PapillaInferior EsophagusSmall IntestineColon

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Kuna, V. K., Kvarnström, N., Elebring, E., Holgersson, S. S. Isolation and Decellularization of a Whole Porcine Pancreas. J. Vis. Exp. (140), e58302, doi:10.3791/58302 (2018).

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