ジャーナル
/
生物工学
/
Isolation and Decellularization of a Whole Porcine Pancreas
JoVE Journal
生物工学
This content is Free Access.
JoVE Journal 生物工学
Isolation and Decellularization of a Whole Porcine Pancreas

Isolation and Decellularization of a Whole Porcine Pancreas

9,108 Views

•

09:08 min

•

October 10, 2018

DOI:

10.3791/58302-v

DOI:
10.3791/58302-v

•

09:08 min
October 10, 2018

•

16 Views
, , ,
1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy,University of Gothenburg, 2The Transplant Institute,Sahlgrenska University Hospital

概要

Automatically generated

Tissue engineering of the whole pancreas is a challenge because of its exocrine and endocrine functions. We show a method for the dissection of an intact porcine pancreas and the process of successful decellularization by perfusion of detergents Triton X-100, sodium deoxycholate, and deoxyribonuclease.

Explore More Videos

Keyword Extraction:Porcine PancreasDecellularizationDissectionBlood VesselsPeristaltic PumpOrgan ChamberDetergent ContainerDetergent Collection ContainerHeparinizedFemale PigMidline IncisionSplenic LobeDuodenal LobeConnection LobeMajor Duodenal PapillaInferior EsophagusSmall IntestineColon

Read Article

Cite this Article

Kuna, V. K., Kvarnström, N., Elebring, E., Holgersson, S. S. Isolation and Decellularization of a Whole Porcine Pancreas. J. Vis. Exp. (140), e58302, doi:10.3791/58302 (2018).

Download .ris file

Copy

Share Video

.

Copy