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生物工程
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Isolation and Decellularization of a Whole Porcine Pancreas
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Isolation and Decellularization of a Whole Porcine Pancreas

Isolation and Decellularization of a Whole Porcine Pancreas

9,108 Views

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09:08 min

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October 10, 2018

DOI:

10.3791/58302-v

DOI:
10.3791/58302-v

•

09:08 min
October 10, 2018

•

16 Views
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1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy,University of Gothenburg, 2The Transplant Institute,Sahlgrenska University Hospital

Summary

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Tissue engineering of the whole pancreas is a challenge because of its exocrine and endocrine functions. We show a method for the dissection of an intact porcine pancreas and the process of successful decellularization by perfusion of detergents Triton X-100, sodium deoxycholate, and deoxyribonuclease.

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Keyword Extraction:Porcine PancreasDecellularizationDissectionBlood VesselsPeristaltic PumpOrgan ChamberDetergent ContainerDetergent Collection ContainerHeparinizedFemale PigMidline IncisionSplenic LobeDuodenal LobeConnection LobeMajor Duodenal PapillaInferior EsophagusSmall IntestineColon

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Kuna, V. K., Kvarnström, N., Elebring, E., Holgersson, S. S. Isolation and Decellularization of a Whole Porcine Pancreas. J. Vis. Exp. (140), e58302, doi:10.3791/58302 (2018).

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