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プライミング多能性胚様体の表面で人間の始原生殖細胞のような細胞の生成誘導多能性幹細胞
JoVE Journal
Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
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プライミング多能性胚様体の表面で人間の始原生殖細胞のような細胞の生成誘導多能性幹細胞

11,942 Views

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12:06 min

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January 11, 2019

DOI:

10.3791/58297-v

DOI:
10.3791/58297-v

•

12:06 min
January 11, 2019

•

4 Views
, , , ,
1Center for Cancer Research,Massachusetts General Hospital, 2Department of Molecular Genetics,Weizmann Institute of Science

Özet

Automatically generated

始原生殖細胞 (PGCs) 精子と卵の両方の共通の前駆体であります。人間の胚の始原生殖は、サイトカインとの相互作用を通じて多能性胚盤葉上層の細胞から指定されます。ここでは、プライミング多能性誘導多能性幹細胞からの胚様体の表面で孵卵に似たひと細胞 transcriptomally を誘導する 13 日間プロトコルについて述べる。

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Mitsunaga, S., Shioda, K., Isselbacher, K. J., Hanna, J. H., Shioda, T. Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells. J. Vis. Exp. (143), e58297, doi:10.3791/58297 (2019).

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