This content is Free Access.
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
11,971 Views
•
12:06 min
•
January 11, 2019
DOI:
Summary
Automatically generated
Primordial germ cells (PGCs) are common precursors of both sperm and eggs. Human embryonic PGCs are specified from pluripotent epiblast cells through interactions of cytokines. Here, we describe a 13-day protocol of inducing human cells transcriptomally resembling PGCs at the surface of embryoid bodies from primed-pluripotency induced pluripotent stem cells.
Read Article
Cite this Article
Mitsunaga, S., Shioda, K., Isselbacher, K. J., Hanna, J. H., Shioda, T. Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells. J. Vis. Exp. (143), e58297, doi:10.3791/58297 (2019).
Copy