Dergi
/
Gelişim Biyolojisi
/
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
JoVE Journal
Gelişim Biyolojisi
This content is Free Access.
JoVE Journal Gelişim Biyolojisi
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

DOI:
10.3791/58297-v

•

12:06 min

•

January 11, 2019

•

, , , ,
1Center for Cancer Research,Massachusetts General Hospital, 2Department of Molecular Genetics,Weizmann Institute of Science

Bölümler

  • 00:04Başlık
  • 01:01Inoculation and Conversion of Primed Pluripotency Human Induced Pluripotent Stem Cells (hiPSCs)
  • 03:40Embryoid Body (EB) Formation Using Microwell Plates
  • 05:52Transfer of EBs to Low Attachment Plates and Generation of hPGCLCs on the Surface of EBs
  • 08:00Immunohistochemical Staining of EBs
  • 09:41Results: Successful Generation of EBs with hPGCLCs on their Surface
  • 11:29Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Primordial germ cells (PGCs) are common precursors of both sperm and eggs. Human embryonic PGCs are specified from pluripotent epiblast cells through interactions of cytokines. Here, we describe a 13-day protocol of inducing human cells transcriptomally resembling PGCs at the surface of embryoid bodies from primed-pluripotency induced pluripotent stem cells.

Etiketler

Human Primordial Germ Cell-like CellsPrimed-pluripotencyInduced Pluripotent Stem CellsGermline ToxicologyGenomic ImpairmentExtracellular MatrixSingle-cell SuspensionNaive Pluripotency4i MediumReproducibilityCell DensityCell Culture Model

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Generation of First Heart Field-like Cardiac Progenitors and Ventricular-like Cardiomyocytes from Human Pluripotent Stem Cells

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Read Article