A long-term culture model of bovine granulosa cells under serum-free conditions is described. This model allows researchers to study the effects of diverse factors and conditions as different plating densities on the characteristics of estrogen-producing bovine granulosa cells.
Baufeld, A., Vanselow, J. A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells. J. Vis. Exp. (139), e58208, doi:10.3791/58208 (2018).