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A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
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Biologie
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Journal JoVE Biologie
A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells

8,660 Views

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05:36 min

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September 06, 2018

DOI:

10.3791/58208-v

DOI:
10.3791/58208-v

•

05:36 min
September 06, 2018

•

8 Views
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1Institute of Reproductive Biology,Leibniz Institute for Farm Animal Biology (FBN)

Tags

Estrogen-producingPrimary Bovine Granulosa CellsCryopreservationCell IsolationFollicular FluidGranulosa CellsCell CultureRNADNAProtein AnalysisCell FreezingCell ThawingAlpha MEM

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Baufeld, A., Vanselow, J. A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells. J. Vis. Exp. (139), e58208, doi:10.3791/58208 (2018).

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