Dergi
/
Nörobilim
/
Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes

Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes

DOI:
10.3791/58116-v

•

10:10 min

•

October 04, 2018

•

, ,
1Groupe de Recherche sur le Système Nerveux Central, and Département de Neurosciences,Université de Montréal, 2Faculté de Médecine Dentaire,Université de Montréal

Bölümler

  • 00:04Başlık
  • 00:30Data Preparation
  • 02:32Cell Counting
  • 04:10Measuring Astrocytic Network Area
  • 05:04Determining the Main Direction Vector
  • 06:52Placing the Analyzed Networks in the Nucleus of Interest
  • 09:11Results: Astrocytic Networks Labeled with Biocytin in NVsnpr
  • 09:49Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here we present a protocol to assess the organization of astrocytic networks. The described method minimizes bias to provide descriptive measures of these networks such as cell count, size, area, and position within a nucleus. Anisotropy is assessed with a vectorial analysis.

Etiketler

Astrocytic NetworksAstrocytesFIJIImageJZ StackMax Intensity ProjectionBackground SubtractionNoise ReductionBinary ImageCell CountingMorphology Analysis

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Isolation and Culture of Mouse Cortical Astrocytes

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration

A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals

Protocol for Three-dimensional Confocal Morphometric Analysis of Astrocytes

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

Three-Dimensional Shape Modeling and Analysis of Brain Structures

Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

Reliable Acquisition of Electroencephalography Data during Simultaneous Electroencephalography and Functional MRI

Live-imaging of Mitochondrial System in Cultured Astrocytes

Read Article