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Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes
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JoVE Journal 神経科学
Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes

Analyzing the Size, Shape, and Directionality of Networks of Coupled Astrocytes

DOI:
10.3791/58116-v

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10:10 min

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October 04, 2018

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, ,
1Groupe de Recherche sur le Système Nerveux Central, and Département de Neurosciences,Université de Montréal, 2Faculté de Médecine Dentaire,Université de Montréal

章

  • 00:04標題
  • 00:30Data Preparation
  • 02:32Cell Counting
  • 04:10Measuring Astrocytic Network Area
  • 05:04Determining the Main Direction Vector
  • 06:52Placing the Analyzed Networks in the Nucleus of Interest
  • 09:11Results: Astrocytic Networks Labeled with Biocytin in NVsnpr
  • 09:49Conclusion

概要

自動翻訳

自動翻訳

Here we present a protocol to assess the organization of astrocytic networks. The described method minimizes bias to provide descriptive measures of these networks such as cell count, size, area, and position within a nucleus. Anisotropy is assessed with a vectorial analysis.

タグ

Astrocytic NetworksAstrocytesFIJIImageJZ StackMax Intensity ProjectionBackground SubtractionNoise ReductionBinary ImageCell CountingMorphology Analysis

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