Functional Evaluation of Olfactory Pathways in Living <em>Xenopus</em> Tadpoles

Beatrice Terni; Paolo Pacciolla; Margalida Perell&#243;; Artur Llobet

doi:10.3791/58028

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Nörobilim

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Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles

JoVE Journal
Nörobilim

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JoVE Journal Nörobilim

Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles

Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles

DOI:

10.3791/58028-v

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07:33 min

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December 11, 2018

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Beatrice Terni*^1,2,3, Paolo Pacciolla*^1,2,3, Margalida Perelló^2,3, Artur Llobet^2,3

¹Laboratory of Neurobiology, Neuroscience Program, Bellvitge Biomedical Research Institute (IDIBELL),L’Hospitalet de Llobregat, ²Department of Pathology and Experimental Therapy, School of Medicine, University of Barcelona,L’Hospitalet de Llobregat, ³Institute of Neurosciences, University of Barcelona,L’Hospitalet de Llobregat

Bölümler

00:04Başlık
00:34Transection of Olfactory Nerves
01:34Labeling of Olfactory Receptor Neurons with Fluorescent Calcium Indicators
02:29Preparation of Tadpoles for Live Imaging of Presynaptic Responses
04:09Live Imaging of Presynaptic Ca²⁺ Changes in Olfactory Glomeruli
04:47Olfactory-guided Behavior Assay
05:43Results: Tracking Olfactory-guided Behavior
07:04Conclusion

Özet

Otomatik Çeviri

Xenopus tadpoles offer a unique platform to investigate the function of the nervous system in vivo. We describe methodologies to evaluate the processing of olfactory information in living Xenopus larvae in normal rearing conditions or after injury.

Etiketler

Olfactory Pathways Xenopus Tadpoles Functional Evaluation Neurobiology Presynaptic Terminals In Vivo Calcium Green Olfactory Bulb Anesthetization Transection Behavioral Experiments Imaging

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