Functional Evaluation of Olfactory Pathways in Living <em>Xenopus</em> Tadpoles

Beatrice Terni; Paolo Pacciolla; Margalida Perell&#243;; Artur Llobet

doi:10.3791/58028

Journal

/

Neurosciences

/

Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles

Journal JoVE
Neurosciences

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Neurosciences

Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles

Functional Evaluation of Olfactory Pathways in Living Xenopus Tadpoles

DOI:

10.3791/58028-v

•

07:33 min

•

December 11, 2018

•

Beatrice Terni*^1,2,3, Paolo Pacciolla*^1,2,3, Margalida Perelló^2,3, Artur Llobet^2,3

¹Laboratory of Neurobiology, Neuroscience Program, Bellvitge Biomedical Research Institute (IDIBELL),L’Hospitalet de Llobregat, ²Department of Pathology and Experimental Therapy, School of Medicine, University of Barcelona,L’Hospitalet de Llobregat, ³Institute of Neurosciences, University of Barcelona,L’Hospitalet de Llobregat

Chapitres

00:04Titre
00:34Transection of Olfactory Nerves
01:34Labeling of Olfactory Receptor Neurons with Fluorescent Calcium Indicators
02:29Preparation of Tadpoles for Live Imaging of Presynaptic Responses
04:09Live Imaging of Presynaptic Ca²⁺ Changes in Olfactory Glomeruli
04:47Olfactory-guided Behavior Assay
05:43Results: Tracking Olfactory-guided Behavior
07:04Conclusion

Summary

Traduction automatique

Xenopus tadpoles offer a unique platform to investigate the function of the nervous system in vivo. We describe methodologies to evaluate the processing of olfactory information in living Xenopus larvae in normal rearing conditions or after injury.

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Appetitive Associative Olfactory Learning in Drosophila Larvae

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

Transplantation of Olfactory Ensheathing Cells to Evaluate Functional Recovery after Peripheral Nerve Injury

Ex Vivo Preparations of the Intact Vomeronasal Organ and Accessory Olfactory Bulb

Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor