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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

11,542 Views

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10:18 min

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October 16, 2018

DOI:

10.3791/58016-v

DOI:
10.3791/58016-v

•

10:18 min
October 16, 2018

•

10 Views
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1Centre for Virus Research, The Westmead Institute for Medical Research,University of Sydney

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Full-length individual proviral sequencing (FLIPS) provides an efficient and high-throughput method for the amplification and sequencing of single, near full-length (intact and defective) HIV-1 proviruses and allows for determination of their potential replication-competency. FLIPS overcomes limitations of previous assays designed to sequence the latent HIV-1 reservoir.

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Hiener, B., Eden, J., Horsburgh, B. A., Palmer, S. Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing. J. Vis. Exp. (140), e58016, doi:10.3791/58016 (2018).

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