Dergi
/
Genetik
/
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
JoVE Journal
Genetik
This content is Free Access.
JoVE Journal Genetik
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

11,579 Views

•

10:18 min

•

October 16, 2018

DOI:

10.3791/58016-v

DOI:
10.3791/58016-v

•

10:18 min
October 16, 2018

•

10 Views
, , ,
1Centre for Virus Research, The Westmead Institute for Medical Research,University of Sydney

Özet

Automatically generated

Full-length individual proviral sequencing (FLIPS) provides an efficient and high-throughput method for the amplification and sequencing of single, near full-length (intact and defective) HIV-1 proviruses and allows for determination of their potential replication-competency. FLIPS overcomes limitations of previous assays designed to sequence the latent HIV-1 reservoir.

Explore More Videos

HIV-1 ProvirusesNext-generation SequencingSingle-cellAmplificationPCRGenetic CompositionReplication CompetentCell TypesLysis BufferSerial DilutionPositive ControlThermal Cycler

Read Article

Cite this Article

Hiener, B., Eden, J., Horsburgh, B. A., Palmer, S. Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing. J. Vis. Exp. (140), e58016, doi:10.3791/58016 (2018).

Download .ris file

Copy

Share Video

.

Copy