Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Bonnie Hiener; John-Sebastian Eden; Bethany A. Horsburgh; Sarah Palmer

doi:10.3791/58016

Journal

/

Génétique

/

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Journal JoVE
Génétique

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Journal JoVE Génétique

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

11,579 Views

•

10:18 min

•

October 16, 2018

DOI:

10.3791/58016-v

DOI:

10.3791/58016-v

•

10:18 min

October 16, 2018

•

10 Views

Bonnie Hiener¹, John-Sebastian Eden¹, Bethany A. Horsburgh¹, Sarah Palmer¹

¹Centre for Virus Research, The Westmead Institute for Medical Research,University of Sydney

Summary

Full-length individual proviral sequencing (FLIPS) provides an efficient and high-throughput method for the amplification and sequencing of single, near full-length (intact and defective) HIV-1 proviruses and allows for determination of their potential replication-competency. FLIPS overcomes limitations of previous assays designed to sequence the latent HIV-1 reservoir.

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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Summary

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