Dergi
/
Nörobilim
/
Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

DOI:
10.3791/57842-v

•

07:16 min

•

August 16, 2018

•

, ,
1Discovery Neuroscience, Pharma Research and Early Development,Roche Innovation Center Basel, 2HiQScreen Sàrl 6

Bölümler

  • 00:04Başlık
  • 00:54Receptor Expression and Recordings in Xenopus Oocytes
  • 02:16Electrophysiological Recordings in Rat Brain Slices
  • 05:39Results: A GABAA NAM Reduces Paired-pulse Inhibition in Hippocampal Slices
  • 06:49Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Here, we present protocols to discover compounds active at GABAA receptors, from the binding to the physiology and pharmacology.

Etiketler

GABA-A ReceptorRadioligand BindingElektrofizyolojiXenopus OocytesRodent Brain SlicesCompound ScreeningSubtype SelectivityEfficacyMicroinjectionVoltage ClampPopulation Spike RecordingHippocampus

Article

Embed

ÇALMA LİSTESİNE EKLE

Usage Statistics

İlgili Videolar

Novel Apparatus and Method for Drug Reinforcement

Application of a NMDA Receptor Conductance in Rat Midbrain Dopaminergic Neurons Using the Dynamic Clamp Technique

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

Methods for Intravenous Self Administration in a Mouse Model

High-resolution Functional Magnetic Resonance Imaging Methods for Human Midbrain

Methods for Studying the Mechanisms of Action of Antipsychotic Drugs in Caenorhabditis elegans

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue

Read Article