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Decellularization and Recellularization Methodology for Human Saphenous Veins
JoVE Journal
Biyomühendislik
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JoVE Journal Biyomühendislik
Decellularization and Recellularization Methodology for Human Saphenous Veins

Decellularization and Recellularization Methodology for Human Saphenous Veins

13,855 Views

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11:35 min

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July 27, 2018

DOI:

10.3791/57803-v

DOI:
10.3791/57803-v

•

11:35 min
July 27, 2018

•

17 Views
, ,
1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy,University of Gothenburg

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Here, we describe a protocol for the saphenous vein decellularization using detergents and recellularization by perfusion of the peripheral blood and the endothelial medium.

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DecellularizationRecellularizationSaphenous VeinTriton-X 100PerfusionWashingBioreactorCell CultureBone MarrowTnBPDNAMedia InletMedia OutletVein InletVein OutletPeristaltic Pump

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Kumar Kuna, V., Xu, B., Sumitran-Holgersson, S. Decellularization and Recellularization Methodology for Human Saphenous Veins. J. Vis. Exp. (137), e57803, doi:10.3791/57803 (2018).

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