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Bioengineering

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JoVE Journal Bioengineering
Decellularization and Recellularization Methodology for Human Saphenous Veins
 

Decellularization and Recellularization Methodology for Human Saphenous Veins

Article DOI: 10.3791/57803-v • 11:35 min • July 27th, 2018
July 27th, 2018 •
1, 1, 1
1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg

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Here, we describe a protocol for the saphenous vein decellularization using detergents and recellularization by perfusion of the peripheral blood and the endothelial medium.

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Decellularization Recellularization Saphenous Vein Tissue Engineering Bioreactor Blood Sample Triton-X 100 Perfusion Washing Decellularization Setup Tube A Tube B Tube C Tube F Tube G Peristaltic Pump Glass Jar
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