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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
JoVE Journal
Genetik
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JoVE Journal Genetik
Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

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11:00 min

June 12, 2018

DOI:

11:00 min
June 12, 2018

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Özet

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This report describes a fast and reliable method for validating mRNA targets of cellular miRNAs. The method uses synthetic biotinylated Locked Nucleic Acid (LNA)-based miRNA mimics to capture target mRNA. Subsequently, streptavidin-coated magnetic beads are employed to pulldown the target mRNA for quantification by qPCR polymerase chain reaction.

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