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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Journal JoVE
Génétique

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Journal JoVE Génétique

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

13,361 Views

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11:00 min

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June 12, 2018

DOI:

10.3791/57786-v

DOI:

10.3791/57786-v

•

11:00 min

June 12, 2018

•

6 Views

Sabyasachi Dash^2,3, Muthukumar Balasubramaniam, Chandravanu Dash, Jui Pandhare

¹School of Biotechnology,Kalinga Institute of Industrial Technology University, ²1. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology,Meharry Medical College, ³Kalinga Institute of Industrial Technology University

Tags

Biotinylated MiRNA Mimics MRNA Targets Pulldown Assay Locked Nucleic Acids Streptavidin-coated Magnetic Beads MiRNA-mRNA Interactions MiRNA Biology MiRNA Therapeutics

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