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线粒体 DNA 甲基化的精确检测方法
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Genetik
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JoVE Journal Genetik
Methodology for Accurate Detection of Mitochondrial DNA Methylation
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线粒体 DNA 甲基化的精确检测方法

13,037 Views

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12:11 min

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May 20, 2018

DOI:

10.3791/57772-v

DOI:
10.3791/57772-v

•

12:11 min
May 20, 2018

•

11 Views
, ,
1The Novo Nordisk Foundation for Basic Metabolic Research, Faculty of Health and Medical Sciences,University of Copenhagen

Özet

Automatically generated

在这里, 我们提出了一个协议, 允许准确定量的线粒体 DNA (线粒体) 甲基化。在本协议中, 我们描述了一种酶消化 DNA 与BamHI结合的生物信息学分析管道, 可用于避免高估线粒体甲基化水平引起的线粒体二级结构。

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Cite this Article

Mechta, M., Ingerslev, L. R., Barrès, R. Methodology for Accurate Detection of Mitochondrial DNA Methylation. J. Vis. Exp. (135), e57772, doi:10.3791/57772 (2018).

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