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Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.' 'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
'Bölümler'
- 00:05'Başlık'
- 00:46Production of Retrovirus and Transduction of MEFs
- 05:01Immunostaining of Reprogrammed MEFs
- 06:44Results: Over-expression of GHMT2m and Inhibition of TGF-β Signaling Increases the Reprogramming Efficiency of MEFs into Functional Cardiomyocytes
- 08:34Conclusion
Here we present a robust method to reprogram primary embryonic fibroblasts into functional cardiomyocytes through overexpression of GATA4, Hand2, Mef2c, Tbx5, miR-1, and miR-133 (GHMT2m) alongside inhibition of TGF-β signaling. Our protocol generates beating cardiomyocytes as early as 7 days post-transduction with up to 60% efficiency.
