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דיכוי של Pro-fibrotic איתות Potentiates בתיווך גורם התכנות של העכבר מתחלקים Fibroblasts לתוך Cardiomyocytes המושרה
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JoVE Journal Gelişim Biyolojisi
Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
Please note that all translations are automatically generated. Click here for the English version.

דיכוי של Pro-fibrotic איתות Potentiates בתיווך גורם התכנות של העכבר מתחלקים Fibroblasts לתוך Cardiomyocytes המושרה

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09:16 min

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June 03, 2018

DOI:

10.3791/57687-v

DOI:
10.3791/57687-v

•

09:16 min
June 03, 2018

•

1 Views
, , , , ,
1Division of Cardiology, Department of Medicine,University of Colorado Anschutz Medical Campus, 2Department of Population Health Sciences, Medical College of Georgia,Augusta University

Özet

Automatically generated

כאן אנו מציגים שיטה חזקה לתכנת fibroblasts עובריים הראשי לתוך cardiomyocytes פונקציונלי. דרך ביטוי של GATA4, Hand2, Mef2c, Tbx5, מיר-1, ומיר-133 (GHMT2m) לצד עיכוב של TGF-β איתות. פרוטוקול שלנו יוצרת מכות cardiomyocytes מוקדם ככל 7 ימים שלאחר התמרה חושית עם עד 60% יעילות.

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Cite this Article

Riching, A. S., Zhao, Y., Cao, Y., Londono, P., Xu, H., Song, K. Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes. J. Vis. Exp. (136), e57687, doi:10.3791/57687 (2018).

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