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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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Genetik
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JoVE Journal Genetik
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

24,474 Views

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09:36 min

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April 10, 2018

DOI:

10.3791/57379-v

DOI:
10.3791/57379-v

•

09:36 min
April 10, 2018

•

37 Views
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1CNRS, CRN2M-UMR7286, Faculté de Médecine Nord,Aix-Marseille Université, 2Plate-forme d’imagerie MRI, UMS3426, CNRS 141,Institut de Génétique Humaine

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This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the lncRNA.

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Torres, M., Becquet, D., Guillen, S., Boyer, B., Moreno, M., Blanchard, M., Franc, J., François-Bellan, A. RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA. J. Vis. Exp. (134), e57379, doi:10.3791/57379 (2018).

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