Journal
/
Genetik
/
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
JoVE Journal
Genetik
This content is Free Access.
JoVE Journal Genetik
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

24,511 Views

•

09:36 min

•

April 10, 2018

DOI:

10.3791/57379-v

DOI:
10.3791/57379-v

•

09:36 min
April 10, 2018

•

37 Views
, , , , , , ,
1CNRS, CRN2M-UMR7286, Faculté de Médecine Nord,Aix-Marseille Université, 2Plate-forme d’imagerie MRI, UMS3426, CNRS 141,Institut de Génétique Humaine

Summary

Automatically generated

This RNA pull-down method allows identifying the RNA targets of a long non-coding RNA (lncRNA). Based on the hybridization of home-made, designed anti-sense DNA oligonucleotide probes specific to this lncRNA in an appropriately fixed tissue or cell line, it efficiently allows the capture of all RNA targets of the lncRNA.

Explore More Videos

RNA Pull-downLong Non-coding RNARNA TargetsRNA RegulationChIRPCHARTBioinformaticsGH4C1 CellsCross-linkingCell CultureLysis Buffer

Read Article

Cite this Article

Torres, M., Becquet, D., Guillen, S., Boyer, B., Moreno, M., Blanchard, M., Franc, J., François-Bellan, A. RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA. J. Vis. Exp. (134), e57379, doi:10.3791/57379 (2018).

Download .ris file

Copy

Share Video

.

Copy