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Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
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April 24, 2018
DOI:
1Animal Ecology, Global Change and Sustainable Development,University of Leuven, 2Systemic Physiological and Ecotoxicological Research,University of Antwerp, 3Water Research Group, Unit for Environmental Sciences and Management,North-West University, 4Centre for Environmental Management,University of the Free State
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In this work, we describe an acute, chronic and multigenerational bioassay to study the effects of single and combined stressors on the Turquoise killifish Nothobranchius furzeri. This protocol is designed to study life-history traits (mortality, growth, fecundity, weight) and critical thermal maximum.
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Turquoise KillifishNothobranchius FurzeriEcotoxicity TestingAcute ToxicityChronic ToxicityLife-history TraitsMaturation TimeGrowthFecundityVertebrate ModelFast Life-cycleMulti-generational EffectsToxicantsTemperatureUV RadiationFood DeprivationPH AlterationsGRZ EggsHatching MediumArtemia NaupliiExposure MediumLC50Chronic Exposure
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Philippe, C., Gregoir, A. F., Thoré, E. S. J., De Boeck, G., Brendonck, L., Pinceel, T. Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri. J. Vis. Exp. (134), e57308, doi:10.3791/57308 (2018).
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