È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
1Animal Ecology, Global Change and Sustainable Development,University of Leuven, 2Systemic Physiological and Ecotoxicological Research,University of Antwerp, 3Water Research Group, Unit for Environmental Sciences and Management,North-West University, 4Centre for Environmental Management,University of the Free State
Capitoli
- 00:04Titolo
- 01:37Hatching of N. furzeri and Short-term Exposure Protocol
- 02:51Chronic Exposure Protocol
- 07:16Results: Ecotoxicity Measurements of Nothobranchius furzeri
- 09:14Conclusion
In this work, we describe an acute, chronic and multigenerational bioassay to study the effects of single and combined stressors on the Turquoise killifish Nothobranchius furzeri. This protocol is designed to study life-history traits (mortality, growth, fecundity, weight) and critical thermal maximum.
Tags
Turquoise KillifishNothobranchius FurzeriEcotoxicity TestingAcute ToxicityChronic ToxicityLife-history TraitsMaturation TimeGrowthFecundityVertebrate ModelFast Life-cycleMulti-generational EffectsToxicantsTemperatureUV RadiationFood DeprivationPH AlterationsGRZ EggsHatching MediumArtemia NaupliiExposure MediumLC50Chronic Exposure
