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脂多糖治疗小鼠肝脏免疫应答中白细胞的双光子活体成像
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver
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脂多糖治疗小鼠肝脏免疫应答中白细胞的双光子活体成像

DOI:
10.3791/57191-v

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07:06 min

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February 06, 2018

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, , ,
1Department of Anatomy,Yonsei University College of Medicine, 2Brain Korea 21 Plus Project for Medical Science,Yonsei University College of Medicine, 3Cerrahi Bölümü,Yonsei University College of Medicine

Bölümler

  • 00:05Başlık
  • 00:28Surgical Process
  • 03:06Intravital Imaging of the Liver with Two-photon MIcroscopy
  • 04:51Results: Two-photon Intravital Imaging in LPS-treated Mouse Liver
  • 06:03Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

我们建立了一种新的小鼠肝脏双光子成像的手术方案。利用这一技术, 我们确定了肝内毒素诱导中的内毒素血症的详细结构。我们预期这种方法可以用来确定各种试剂治疗肝白细胞迁移的有效性。

Etiketler

Two-photon Intravital ImagingLeukocytesImmune ResponseLipopolysaccharideMouse LiverSeptic ShockLiver SurgeryAnesthesiaTexas Red DextranPovidone IodineAbdominal CavityLiver ExposureIntravital ImagingLaser SettingsWater Immersion Objective LensTemperature Control

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