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Absorción de Metal esencial en bacterias Gram-negativas: rayos x de fluorescencia, radioisótopos y fraccionamiento de la célula
JoVE Journal
Kimya
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JoVE Journal Kimya
Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation
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Absorción de Metal esencial en bacterias Gram-negativas: rayos x de fluorescencia, radioisótopos y fraccionamiento de la célula

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10:34 min

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February 01, 2018

DOI:

10.3791/57169-v

DOI:
10.3791/57169-v

•

10:34 min
February 01, 2018

•

7 Views
, , , , ,
1Graduate Biomedical Sciences Microbiology Theme,University of Alabama at Birmingham, 2Department of Radiology,University of Alabama at Birmingham, 3Office of the Provost,University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology,University of Alabama at Birmingham

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Un protocolo para la extracción de una chaperona de periplasmic metales de transición en el contexto de sus socios de Unión nativos y caracterización biofísica de su contenido de sustrato mediante rayos x se presenta absorción fluorescencia y radiometal.

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Cite this Article

Radka, C. D., Radford, L. L., Massicano, A. V., DeLucas, L. J., Lapi, S. E., Aller, S. G. Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation. J. Vis. Exp. (132), e57169, doi:10.3791/57169 (2018).

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