Journal
/
化学
/
Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation
JoVE 杂志
化学
This content is Free Access.
JoVE 杂志 化学
Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation

DOI:
10.3791/57169-v

•

10:34 min

•

February 01, 2018

•

, , , , ,
1Graduate Biomedical Sciences Microbiology Theme,University of Alabama at Birmingham, 2Department of Radiology,University of Alabama at Birmingham, 3Office of the Provost,University of Alabama at Birmingham, 4Department of Pharmacology and Toxicology,University of Alabama at Birmingham

Chapters

  • 00:04Title
  • 01:09Cell Fractionation and Protein Crystallization
  • 03:52X-ray Fluorescence (Using GM/CA Beamline Software)
  • 04:44MAD Scan for Metal Energy Absorption Peak (Using GM/CA Beamline Software)
  • 05:45Analytical Protocol for Radioactive Metal Uptake Assay
  • 07:24Results: Extraction of Bacterial Periplasm from Cytoplasm in Gram-negative Bacteria
  • 09:14Conclusion

Summary

自动翻译

自动翻译

A protocol for the extraction of a periplasmic transition metal chaperone in the context of its native binding partners, and biophysical characterization of its substrate contents by X-ray fluorescence and radiometal uptake is presented.

Tags

Keywords: Metal UptakeGram-negative BacteriaX-ray FluorescenceRadioisotopesCell FractionationABC Transport SystemsSubstrate BindingPeriplasmOsmotic ShockSpheroplastsProtein Extraction

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/57169?article_type=v

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
check_url/cn/57169?article_type=v

Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
check_url/cn/57169?article_type=v

X-ray Powder Diffraction in Conservation Science: Towards Routine Crystal Structure Determination of Corrosion Products on Heritage Art Objects
check_url/cn/57169?article_type=v

Quantifying X-Ray Fluorescence Data Using MAPS
check_url/cn/57169?article_type=v

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography
check_url/cn/57169?article_type=v

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering
check_url/cn/57169?article_type=v

Ligand-Mediated Nucleation and Growth of Palladium Metal Nanoparticles
check_url/cn/57169?article_type=v

Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
check_url/cn/57169?article_type=v

Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
check_url/cn/57169?article_type=v

Biological Samples Preparation for Speciation at Cryogenic Temperature using High-Resolution X-Ray Absorption Spectroscopy

Read Article